Question: Why is using diluted Nuclei Buffer for resuspending my nuclei important?
Answer: The use of the diluted Nuclei Buffer for nuclei suspension is critical for optimal Single Cell ATAC assay performance. The composition of the Tris-based Diluted Nuclei Buffer, including magnesium concentration, has been optimized for the Transposition and Barcoding steps in the Single Cell ATAC protocol. Suspension of nuclei in a different buffer may not be compatible with 10x assay.
Please note that the Nuclei Buffer (PN-2000153) is provided as a concentrated 20X stock solution as part of the Single Cell ATAC Library and Gel Bead Kit, and a diluted working stock (1X) must be used for resuspending nuclei in the last step of the Nuclei Isolation for Single Cell ATAC Sequencing Demonstrated Protocol (CG000169).
Products: Single Cell ATAC