Question: How much of a single cell's genome is amplified?
Answer: For human samples, approximately 0.20x of each cell's genome is amplified. However, not all of these library fragments are sequenced. At 750,000 read pairs per cell (human), approximately 0.05x coverage per cell is sequenced. This is enough to perform CNV calling at 2 Mb resolution at the single cell level, but not to reliably detect smaller CNV events or SNPs.
To sequence all of the amplified fragments, a much higher sequencing depth (>>750,000 read pairs per cell) is required. There are diminishing returns to sequencing to higher depths because many more duplicate reads are sequenced as a fraction of total reads.
Products: Single Cell DNA