Question: What if I don't have enough Cell Beads to load onto Chip D?
Answer: If you do not have 30 ul of Cell Beads to load in Chip D (Step 3.2d), it is possible to load less. Specifically:
- If you have between 25-30 ul of Cell Beads, load entire volume in Chip D.
- If you have less than 25 ul of Cell Beads, bring total volume up to 25 ul with CB buffer, pipette mix, and load entire volume in Chip D.
Note: If starting with less than 25 ul of Cell Beads to load in Chip D, the total number of recovered cells will be less than targeted.
Products: Single Cell DNA