Question: What alternative cell suspension buffers can be used?
Answer: For the Single Cell DNA workflow, the following buffers and media have been tested and are compatible:
–1X PBS + 0.04% BSA
–HBSS + 0.04% BSA
–EMEM + 10% FBS
–DMEM + 10% FBS
–RPMI + 10% FBS
–Hams F12 + 10% FBS
–IMDM + 10% FBS
–DMEM:F12 + 10% FBS
Note: Higher levels of BSA (>0.04%) or FBS (>10%) will negatively impact assay performance and are not recommended.
Products: Single Cell DNA