Question: Does 10x have a demonstrated protocol for isolating PBMCs?
Answer: Unfortunately, at 10x we do not have a protocol for isolating PBMCs. We purchase fresh human PBMCs from AllCells (https://www.allcells.com/products/peripheral-blood-mononuclear-cells-pbmc). AllCells collect mononuclear cells with a clinical-grade apheresis system (Spectra Optia® Apheresis System) and subsequently deplete RBCs and platelets with ammonium chloride and centrifugation, respectively.
Customers have also reported success using Heparin tubes for blood sampling. We have not tested this in-house, but if using this solution, cells should be washed to remove any residual contaminants with PBS + 0.04% BSA and still perform an RBC lysis step. Using the highest concentration of cells that go into the sample master mix minimizes carryover of residual heparin.
Products: Single Cell Gene Expression, Single Cell Immune Profiling