Question: Does 10x have a demonstrated protocol for isolating PBMCs?
Answer: Instructions for isolating PBMCs can be found in the Isolation of Leukocytes, Bone Marrow and Peripheral Blood Mononuclear Cells for Single Cell RNA Sequencing Demonstrated Protocol (CG000392).
The following collection tubes and isolation methods have been validated for use with 10x Genomics single cell assays:
Products: Single Cell Gene Expression, Single Cell Immune Profiling