Question: How do I extract genomic DNA from bacteria?
Answer: We do not have in-house experience with bacterial genomic DNA preps, but would recommend starting with the MagAttract HMW DNA Kit from Qiagen.
This kit has a modification option available for gram-negative and gram-positive bacteria in the protocol. Any method that gives HMW gDNA such as MagAttract, or any method used for BAC isolation or recommended for a long read sequencing platform should be suitable for the 10x Genome workflow.
Products: Genome