Question: Is there a recommended protocol for shipping cells?
Answer: The preferred method for shipping samples is by shipping cryopreserved single cell suspensions on dry ice. Post-thaw, there will be a decrease in viability and cell recovery, however the degree of this will primarily depend on the cell type and how the cells were cryopreserved.
10x Demonstrated Protocols for cryopreservation can be found here:
- PBMC samples: https://support.10xgenomics.com/single-cell-gene-expression/sample-prep/doc/demonstrated-protocol-fresh-frozen-human-peripheral-blood-mononuclear-cells-for-single-cell-rna-sequencing
- Cell lines: https://support.10xgenomics.com/single-cell-gene-expression/sample-prep/doc/demonstrated-protocol-fresh-frozen-human-mouse-cell-line-mixtures-for-single-cell-rna-sequencing
Other sample types should be cryopreserved in an appropriate freezing medium.
Cells should be thawed and washed prior to use (see thawing instructions in the protocols above).
Products: Single Cell 3', VDJ, Single Cell DNA