Question: Can I generate a matrix h5 file from a molecule_info.h5 file?
Answer: Yes, the molecule_info.h5 file contains all molecule information and can be used to re-generate the gene-barcode matrix files.
Although the main purpose of the
cellranger aggr pipeline is to combine multiple 10x libraries in a single analysis, it could also be used to analyze data from a single library with a molecule_info.h5 file.
You would provide the path to the molecule info file in the CSV input file for
The pipeline will generate the matrix h5 files, .cloupe file and the web summary HTML file.