Question: How can I ship cells?
Answer: We recommend shipping cryopreserved cells on dry ice with enough dry ice to prevent cells from thawing.
After shipping and thawing the cells, we expect some decrease in viability and total cell recovery. The degree will primarily depend on the cell integrity and cryopreservation conditions.
For more information on how to cryopreserve cells, we have the following Demonstrated Protocols:
1. Fresh frozen human peripheral blood mononuclear cells for single cell RNA sequencing:
2. Fresh frozen human-mouse cell line mixtures for single cell RNA sequencing:
Other useful guidelines:
- We recommend freezing at least one million cells to ensure a sufficient number will be recovered post-thaw. It is expected that you will lose almost half from the freeze-thaw and during the wash and centrifugation steps.
-After thawing, cells should be washed and filtered (optional, recommended if debris and/or clumps are visible) and the final cell suspension counted prior to loading.
Products: Single Cell 3', VDJ