Question: Can you run Single Cell 5' and 3' samples on the same Single Cell A Chip?
Answer: It depends on the Single Cell 3' chemistry version. If running Single Cell 3' v2 reagents, then both Single Cell 5' and 3' samples can be run on the same Chromium Chip A. Critical: the Gel Beads and reagents in the Master Mixes are different between the two workflows and cannot be interchanged. Therefore, two different Gel Bead strips (5' and 3') and two different Master Mixes must be made and loaded into the chip.
If running Single Cell 3' v3 reagents, a different chip must be used. Single Cell 3' v3 workflow is only compatible with Chromium Chip B.
Products: Single Cell 3, VDJ