Question: What is the maximum number of cells that can be profiled?
Answer: In each 10x Single Cell Gene Expression, Immune Profiling, ATAC, or multiome library, it is possible to target up to 10,000 cells, thus loading ~17,400 cells. We do not recommend targeting more than 10,000 cells per library because this increases the sample's likelihood of clogging the microfluidic channel in the chip. Each chip can process eight libraries in parallel, allowing users to target up to 80,000 cells per chip.
An important consideration when deciding how many cells to target is the multiplet rate or the mean fraction of cell-associated barcodes estimated to be associated with more than one cell. The multiplet rate increases linearly with the number of cells loaded:
You can control the multiplet rate by adjusting the number of cells that are loaded, and consequently, the number of recovered cells.
Products: Single Cell Gene Expression, Single Cell Immune Profiling, Single Cell ATAC, Genome/Exome, Single Cell CNV, Connect, Single Cell Multiome ATAC+GEX