Question: Why do I need to use qPCR to quantify my final library?
Answer: We recommend using the Kapa DNA Quantification Kit to measure the final concentration of your 10x library.
Kapa detects diverse DNA fragments with similar efficiency, and is the method of choice for library measurements due to its sensitivity as well as its ability to specifically quantify sequenceable library molecules, i.e., those fragments that contain both the Illumina p5 and p7 sequences at the ends of the molecules. Bioanalyzer measurements do not have enough accuracy for sequencing, and both Bioanalyzer and Qubit methods measure all nucleic acid, including fragments that are not sequenceable.
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