Question: Is it possible to combine SC3' v2 gene expression and SC5' gene expression libraries on a sequencing run?
Answer: Yes. It is possible to combine SC3' v2 gene expression and SC5' gene expression libraries on a single lane in a sequencing run as the read formats are the same.
|Read||Read 1||i7 Index||i5 Index||Read 2|
|Purpose||10x™ Barcode, UMI||Sample Index||N/A||Insert|
****** Shorter Read 2 reads may lead to reduced transcriptome alignment rates. 10x™ Barcode, UMI, and Sample index reads must not be shorter than indicated. Any read can be longer than recommended. Additional bases in Sample index reads must be trimmed using cellranger mkfastq or Illumina's bcl2fastq prior to further analysis.
For more information, please refer to the sequencing requirements for both 3' and 5' solutions:
Products: Single Cell Gene Expression Solution, Single Cell Immune Profiling Solution