Question: Can I store purified cDNA beyond 1 week at -20°C?
Answer: For Single Cell 3' v2 and Single Cell 5' workflows, we have not tested storing purified cDNA beyond 1 week at -20°C. It is likely safe to store the amplified cDNA for up to two weeks, but this is untested. The risk of storing it longer is that there will be a lower number of genes detected per cell if the cDNA is degraded. The safest thing to do is to process the amplified cDNA within 1 week of storage at -20°C.
For Single Cell 3' v3, it is possible to store purified cDNA for up to 4 weeks at -20°C.
Products: Single cell 3', VDJ