Question: Are cardiomyocytes compatible with Single Cell assays?
Answer: We have not tried processing cardiomyocytes internally, but have had a number of customers attempt to process them with various success rates. The microfluidic channels are approximately 50 um in diameter, so very long cells such as cardiomyocytes have the potential to clog the channels.
One important distinction is whether the cardiomyocytes are from adult or embryonic samples, as well as the organism. Some customers have successfully generated GEMs and amplified cDNA from mouse embryonic heart tissue. However, with older mice and adult patient cardiomyoctes, the larger size of the cells may clog the microfluidic channels on the chip. For adult cardiomyocytes, nuclear isolation may be a more reliable way to run these samples.
Our Demonstrated Protocol for Isolation of Nuclei for Single Cell RNA Sequencing can be found here: https://support.10xgenomics.com/single-cell-gene-expression/sample-prep/doc/demonstrated-protocol-isolation-of-nuclei-for-single-cell-rna-sequencing
Products: Single Cell 3', VDJ