Question: Are RNase inhibitors required in the preparation of my sample?
Answer: We recommend using RNase inhibitors when preparing cells samples that have been fixed with methanol, or granulocytes(that are rich in RNases) as detailed in our Best practices for working with granulocytes and Demonstrated Protocols for Methanol Fixation of Cells for Single Cell RNA Sequencing.
For nuclei samples, it is mandatory to use RNAse inhibitor in sample prep. For the 3'GEX assay, we recommend a concentration of 0.2U/ul and for the Multiome assay, we recommend a concentration of 1U/ul in all buffers.
We have tested Protector RNase inhibitor (Sigma Aldrich PN-3335399001) and found that it gives the best results. Note that some RNAse inhibitors may negatively affect your experiment. Please see: Can I use an alternative RNase inhibitor part number?
Products: Single Cell Gene Expression, Single Cell Multiome ATAC + GEX