Question: Can RNase inhibitors be used in the preparation of my sample?
Answer: We recommend using RNase inhibitors when preparing nuclei suspensions or cells that have been fixed with methanol, as detailed in our Demonstrated Protocols for Isolation of Nuclei for Single Cell RNA Sequencing and Methanol Fixation of Cells for Single Cell RNA Sequencing.
We have tested RNase inhibitor (Sigma Aldrich PN-3335399001) concentrations up to 1.0 U/µl and did not see degradation in 10x assay performance.
Note: RNase Inhibitor in the Nuclei Washing and Resuspension Buffer or Rehydration Buffer may result in a translucent/opaque rather than clear aqueous phase after breaking the GEMs with Recovery Agent. This is normal and will not impact downstream operations.
Products: Single Cell 3'