Question: Can InTraSeq™ reagents be used with 10x Genomics Chromium Single Cell 3' Kits with Feature Barcode technology?
Answer: The InTraSeq™ method, launched by Cell Signaling Technology (CST), enables researchers to measure proteins (cytoplasmic, surface, and nuclear), including post-translational modifications, alongside RNA in single cell assays.
CST has validated InTraSeq™ 3' Conjugates/Antibody Cocktails and the InTraSeq™ 3' Assay Kit with 10x Genomics Chromium Single Cell 3' Kits with Feature Barcode technology. The protocol tested by CST can be found here: InTraSeq™ 3' Protocol.
10x Genomics has not tested nor validated InTraSeq™ reagents. 10x Genomics cannot guarantee assay performance and cannot provide troubleshooting guidance for optimizing InTraSeq™ experiments. For technical inquiries, please contact CST technical support at support@cellsignal.com.
Products: Single Cell Gene Expression