Question: I have been running experiments using the pulldown-based Targeted Gene Expression assay. I would like to continue my project and order more baits for additional genes. How can I do it?
Answer: As of June 30, 2023, we have discontinued the sale of our Targeted Gene Expression products and access to the online Custom Panel Designer Tool. We suggest exploring other innovative products within our portfolio that could meet your research needs.
If you still want to pursue the similar hybridization capture method, although not tested or supported by 10x Genomics, you can procure custom probes and alternative kits from third party providers following their manufacturing protocols.
If you prefer to use the 10x Genomics pre-designed baits, information on human panels is available on our support page: https://support.10xgenomics.com/targeted-gene-expression/panel-selection/overview. If you are looking to include additional human or mouse genes, you can access the bait design information for all human and mouse genes via the provided links:
- Human: BED file, Target Panel CSV file
- Mouse: BED file, Target Panel CSV file
Please note that recent versions of Cell Ranger and Space Ranger no longer support targeted gene expression analysis. For analyses involving targeted gene expression libraries, we recommend using Cell Ranger version 7.1.0 or earlier, or Space Ranger version 2.1.1 or earlier.
Updated: Jun 14, 2024