Question: Is the Cq value I obtained during the Cycle Number Determination Step (6.1) of the Visium HD assay workflow within the expected range?
Answer: Cq values are influenced by tissue sample quality, size, composition, and workflow steps, therefore, it is difficult to provide an expected range. We’ve found that when the Cq value is >15, this generally indicates that there may have been a workflow error/issue leading to lower ligated probe product generated than expected. This will likely lead to a drop in assay sensitivity.
If the tissue is very small, not very complex (e.g., contains high amounts of connective tissue), of poor quality higher Cq values (15-20) may be expected.
For samples with Cq >20, we would not recommend proceeding as likely a workflow error occurred; please contact support@10xgenomics.com for assistance.
Products: Visium HD