Question - How do I select the correct library type for my experiment in the Cloud Analysis UI?
Answer - If you have uploaded your FASTQ files successfully, the files have automatically been grouped into sets upon upload. To determine which pipeline is appropriate for your data, you must specify the library or feature type corresponding to the FASTQ sets that you are analyzing.
You may assign values one at a time using the dropdown selectors contained in each row of the table, highlighted in the screenshot below.
Note that you will additionally need to pay close attention to select GEM-X or Next GEM single cell gene expression chemistries as applicable to your samples. For instance, if you have samples from the 3' GEX v3 assay or the 5' GEX v2 assay, select the library type starting with 'Next GEM Single Cell 3' Gene Expression' or 'Next GEM Single Cell 5' Gene Expression, respectively'.
If your samples are from 3' GEX v4 assay or 5' GEX v3 assay which are new in 2024, then you will select GEM X Single Cell 3' Gene Expression or GEM X Single Cell 5' Gene Expression, respectively.
Products: Single Cell Gene Expression, Single Cell Immune Profiling
Last Updated: Apr 26, 2024