Question: What sequencing configuration is needed for Chromium GEM-X Immune Profiling 5’ v3?
Answer: Our recommendations for sequencing configuration and minimum sequencing depth are listed in the sequencing section of each assay User Guide. Please ensure that the correct User Guide is used for the assay.
For 5' v3 we recommend the following sequencing configuration for Gene Expression, V(D)J, Cell Surface Protein, and CRISPR libraries:
Read 1 requires 28 cycles with 5' v3 libraries as the UMI length on the Gel Bead oligo is now 12 nt instead of 10 nt.
Products: Single Cell Immune Profiling