Question: Is it expected to see a large pellet of lyophilized Template Switch Oligo B (PN-2001027) used with Chromium GEM-X Single Cell 3’ v4?
Answer: Yes, it is an expected phenotype. The pellet may be larger in size than what users may be used to when working with Template Switch Oligo (PN-3000228) used in the Chromium Next GEM Single Cell 3’ v3.1 assay.
Please follow the User Guide instructions for reconstitution: Centrifuge briefly, resuspend in 65 µl Low TE Buffer. Vortex 15 sec at maximum speed, centrifuge briefly. The resuspended solution can be used immediately. After resuspension, store at −80°C. Thaw at room temperature for ≥ 30 minutes in subsequent uses.
Ensure the lyophilized pellet is fully resuspended before proceeding with the workflow.
Products: Single Cell Gene Expression