Question: Does the cell segmentation add-on kit work with organisms other than human and mouse?
Answer: Xenium Cell Segmentation Staining Reagents have been validated on human and mouse tissues (Tested Tissue List). The Multi-Tissue Stain Mix contains human and mouse specific antibodies. While there is a likelihood that some of these stains will perform well in other closely related species, such as rat or non-human primates, this has not been tested. Stain intensity may vary depending on the species of interest, tissue cell-type composition, and tissue quality. In addition, each component of the Multi-tissue Stain Mix may not perform comparably across species. For example, boundary stain may be strong in Species 1 while membrane stain is weak in Species 1.
Homology predictions for 18s indicate theoretical stain compatibility and functionality in pig, macaque, rat, and possibly Zebrafish. This said, for any non-human or non-mouse species (e.g. non-human primates, rodents, etc.), we would recommend a pilot to confirm compatibility.
The Multi-Tissue Stain Mix is incompatible with Drosophila and plants.
Product: Xenium In Situ Gene Expression, Multimodal Cell Segmentation