Question: Does the cell segmentation add-on kit work with organisms other than human and mouse?
Answer: Xenium Cell Segmentation Staining Reagents have been validated on human and mouse tissues (Xenium In Situ Multimodal Cell Segmentation:
Workflow and Data Highlights and Tested Tissue List). he Multi-Tissue Stain Mix contains human and mouse specific antibodies. While there is a likelihood that some of these stains will perform well in other closely related species, such as rat or non-human primates, this has not been tested. Stain intensity may vary depending on the species of interest, tissue cell-type composition, and tissue quality. In addition, each component of the Multi-tissue Stain Mix may not perform comparably across species. For example, relative intensity of the boundary stain may vary across different species .
Homology predictions for 18s indicate theoretical stain compatibility and functionality in pig, macaque, rat, and possibly Zebrafish.
Here we see homology predictions of n=16 Xenium 18s RNA detection probe sequences blast against Macaque, Rehsus, Rat, Pig, and Zebrafish genomes. Even Zebrafish has high identity for some of the probes (in green). This said, for any non-human or non-mouse species (e.g. non-human primates, rodents, etc.), we would recommend a pilot to confirm compatibility.
The Multi-Tissue Stain Mix is incompatible with Drosophila and plants.
Product: Xenium In Situ, Multimodal Cell Segmentation