Question: Can additional antibodies or alternative stains be spiked into the multi-tissue cocktail?
Answer: No. Adding antibodies or additional molecular labels to the multi-tissue stain mix is officially unsupported. Modifying the multi-tissue stain mix risks the following:
- Decreased assay performance of both the core GEX assay as well as Multimodal Cell Segmentation.
- No read-outs from foreign antibodies during the Xenium run as these are incompatible with Detection Reagents.
If additional antibody outputs are desired, we would recommend proceeding with Post-Xenium Immunofluorescence (CG000709).
Products: Xenium In Situ Gene Expression, Multimodal Cell Segmentation