Question: Is there a safe stopping point between the Post-Ligation Cleanup and SI-PCR steps?
Answer: We do not recommend stopping between the Post-Ligation Cleanup and the SI-PCR steps. At this stage, the DNA fragments with ligated adaptor have not been amplified and any degradation that occurs during storage could adversely affect assay performance.
If possible, proceed through the SI-PCR step and store the PCR-amplified product at 4°C for up to 72 h.
Products: Single Cell 3', VDJ