Question: Can GEMs be stored after isothermal incubation?
Answer: Yes, the GEMs can be stored after isothermal incubation at 4°C for up to 72 h or at -20°C for up to 1 week before proceeding to Post-GEM incubation cleanup.
The product of the isothermal incubation is a mixture of double-stranded and single-stranded DNA products. Subsequent end repair step will fix ends of double-stranded and partially double-stranded products, while purely single-stranded molecules are likely not to be repaired or converted.
Product: Single Cell 3', VDJ