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Which thermal cyclers are supported for use with GEMs?

Question: Which thermal cyclers are supported for use with GEMs?

Answer: The thermal cyclers listed in the table below have been validated by 10x Genomics for Single Cell assays. Please also refer to the Supported Thermal Cyclers web page. 

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In addition to the thermal cyclers listed in the table above, we have also validated the following thermal cyclers that are now discontinued:

  • Thermo Fisher Scientific - Veriti 96 well Thermal Cycler, 4375786 (discontinued)
  • Eppendorf Mastercycler Pro, North America 950030010, International 6321 000.019 (discontinued)
    • Supported for Single Cell Gene Expression, Single Cell Immune Profiling, Single Cell ATAC, Single Cell Multiome ATAC + GEX
    • Has not been tested for Single Cell Flex / Fixed RNA Profiling.

If none of the above models are available, the minimum specifications are required:

  • The GEM emulsion volumes should be within the capacity of the thermal cycler
  • The thermal cycler supports uniform temperature cycling
  • The thermal cycler has a temperature-controlled lid
  • The thermal cycler should include either a deep-well or a 0.2 ml block configuration
  • The thermal cycler must allow the block to begin cycling before the lid temperature has reached the desired temperature. This prevents extended delays in block cycling in thermal cycler protocols where the lid needs to cool down to a specific temperature.

Please check with the thermal cycler manufacturer to ensure the above specifications are met. Assay performance cannot be guaranteed if an unsupported thermal cycler is used. See: What metrics could be affected by using an unsupported thermal cycler to prepare single cell gene expression libraries?

 

Products: Single Cell Gene Expression, Single Cell Immune Profiling, Single Cell ATAC, Single Cell Multiome ATAC + GEX, Single Cell Gene expression Flex