Question: How should I count my cells?
Answer: We recommend the following cell counting methods:
- Countess II Automated Cell Counter (Thermo Fisher), cell size range 7-60 um, limited in measuring viability of very small cells
- Hemocytometer, any cell size range
- Scepter Handheld Automated Cell Counter (Millipore), cell size range 3-18 um and 8-25 um, limited dynamic range
Accurate cell counting is critical to application success.
It is recommend the single cell suspension be between 700 - 1200 cells/µl for optimal target cell recovery. The cell suspension should be counted at least 3-4 times and the average of all measurements should be taken. The standard deviation between all counts should not be > 25%.
A flowchart outlining the recommended sample preparation procedure can be found at: https://support.10xgenomics.com/single-cell-gene-expression/sample-prep/doc/technical-note-chromium-single-cell-applications-guidelines-for-optimal-sample-preparation
For additional information on obtaining accurate cell counts, see the related Technical Note (https://support.10xgenomics.com/single-cell-gene-expression/index/doc/technical-note-guidelines-for-accurate-target-cell-counts-using-10x-genomics-single-cell-solutions)
Products: Single Cell 3', VDJ