Question: How do I extract genomic DNA from fungal samples?
Answer: We don't have experience extracting HMW DNA from fungal samples, but since the Qiagen MagAttract HMW kit typically gives very high-quality DNA, we suggest processing fungal samples with this kit and the steps below:
- Prepare (and lyse) spheroplasts from fungus.
- Use the number of fungal cells required to achieve a DNA mass equivalent to the mass of DNA extracted from 1,000,000 human cells (~6.6 ug) for each prep, or test a titration (underloading cells results in shorter DNA molecules).
- Bind, wash, and elute the fungal DNA.
Products: Genome, Exome