Question: What is the recommended loading concentration of Visium CytAssist Spatial Gene and Protein Expression libraries on the NovaSeq 6000 Illumina sequencing platform?
Answer: To improve sequencer output, we’ve adjusted our loading concentration so users can maximize their sequencing runs. In some cases, for NovaSeq 6000, we have observed loading concentration outside of our recommended range can lead to poor run performance, lower Q30 scores, possible introduction of sequencing artifacts, and, counterintuitively, lower total data output (i.e., fewer usable reads) as compared to optimally loaded runs. To improve the robustness of sequencing runs, we are updating our loading recommendations for the NovaSeq 6000 as follows:
· NovaSeq 6000 Standard Workflow: 100 pM – 150 pM
· NovaSeq 6000 XP Workflow: 150 pM – 200 pM
We will be revising the User Guides to include this updated guidance for the NovaSeq 6000 sequencing platform. For other Illumina sequencers, the recommended loading concentrations remain unchanged. For additional information regarding sequencing requirements for Visium CytAssist Spatial Gene Expression libraries, please see here. For Visium CytAssist Spatial GEX and Protein libraries, please see here.
Products: Visium CytAssist for FFPE, Visium CytAssist for FF, Visium CytAssist for FxF, Visium CytAssist Gene and Protein