Question: When should I start to incorporate the thermal cycler protocol adjustment (updating Pre-Amplification annealing temperature)?
Answer: When performing side-by-side testing using either 67°C or 63°C for the Pre-Amplification annealing temperature with experiments using on-market products (Singleplex, Singleplex with Feature Barcoding (using TotalSeq™️-B), and Multiplex) generated comparable results regarding library quality and complexity.
Therefore, users can either wait to complete on-going experiments within a study or immediately migrate to the new annealing temperature without concerns about introducing batch effects due to this change.
Product: Fixed RNA Profiling, Single Cell Gene Expression Flex