Question: What happens if I forget to update my thermal cycler program for Pre-Amplification and I perform Barcode Oligo Capture protocols?
Answer: TotalSeq™️-C Feature Barcode library yield will be less and there will be slightly reduced complexity when using the higher annealing temperature (67°C rather than 63°C) but users should still be able to generate good-quality Feature Barcode libraries while using the higher annealing temperature.
Product: Fixed RNA Profiling, Single Cell Gene Expression Flex