Question: What 10x kits are required for performing Fixed RNA Profiling with Feature Barcode technology for Protein using Barcode Oligo Capture (TotalSeq™️-C compatible)?
Answer: The following kits are required for Singleplexed experiments:
| Product Description | Part Number |
| Chromium Next GEM Single Cell Fixed RNA Sample Preparation Kit, 16 rxn | 1000414 |
| Chromium Fixed RNA Kit, Human Transcriptome, 4 rxns x 1 BC | 1000474 |
| Chromium Fixed RNA Kit, Mouse Transcriptome, 4 rxns x 1 BC | 1000495 |
| Chromium Next GEM Chip Q Single Cell Kit, 16 rxns | 1000422 |
| Chromium Next GEM Chip Q Single Cell Kit, 48 rxns | 1000418 |
| Fixed RNA Feature Barcode Multiplexing Kit, 64 rxn* | 1000628 |
| Dual Index Kit TS Set A, 96 rxns | 1000251 |
| Dual Index Kit TN Set A, 96 rxns | 1000250 |
*The Feature Barcode Multiplexing Kit is still required for Singleplexed assays, when performing Feature Barcoding using TotalSeq™️-C.
The following kits are required for Multiplexed experiments:
| Product Description | Part Number |
| Chromium Next GEM Single Cell Fixed RNA Sample Preparation Kit, 16 rxn | 1000414 |
| Chromium Fixed RNA Kit, Human Transcriptome, 4 rxns x 4 BC |
1000475
|
| Chromium Fixed RNA Kit, Mouse Transcriptome, 4 rxns x 4 BC | 1000496 |
| Chromium Fixed RNA Kit, Human Transcriptome, 4 rxns x 16 BC | 1000476 |
| Chromium Fixed RNA Kit, Mouse Transcriptome, 4 rxns x 16 BC | 1000497 |
| Chromium Next GEM Chip Q Single Cell Kit, 16 rxns | 1000422 |
| Chromium Next GEM Chip Q Single Cell Kit, 48 rxns | 1000418 |
| Fixed RNA Feature Barcode Multiplexing Kit, 64 rxn | 1000628 |
| Dual Index Kit TS Set A, 96 rxns | 1000251 |
| Dual Index Kit TN Set A, 96 rxns | 1000250 |
Product: Fixed RNA Profiling, Single Cell Gene Expression Flex