Question: At what concentration should standalone ATAC or Multiome ATAC libraries be loaded for the NovaSeq 6000 XP workflow?
Answer: Both standalone and Multiome ATAC libraries should be loaded at a concentration of 150pM for the NovaSeq 6000 XP (lane splitting) workflow. We strongly recommend quantifying libraries using KAPA Library Quantification Kits to determine optimal library concentration prior to Illumina sequencing (see How do alternative quantification methods other than qPCR impact library loading?).
Products: Single Cell ATAC, Single Cell Multiome ATAC+GEX