Question: What is the Median Normalized Average statistic in Loupe Browser in the Moran's I table?
This statistic is used during "Moran's I" analysis in Loupe Browser. The median-normalized average (MNA) of a gene is calculated as the mean of observed UMI counts normalized by the size factor for each cell in the tissue-covered data. It is calculated as follows:
MNA(gene_X) = mean ([gene_X_UMI_count / size_factor for spots under tissue])
where the size factor is calculated as follows:
size_factor = (total_UMI_counts_in_current_spot/median_UMI_counts_per_spot)