Question: Can I ship fixed single cell/nuclei samples for use with the Fixed RNA Profiling Assay?
Answer: Yes, fixed single cell/nuclei samples can be shipped for use with the Fixed RNA Profiling Assay (i.e., Single Cell Gene Expression Flex assay).
Short Term Storage:
Fixed single cell/nuclei samples can be resuspended in Quenching Buffer and Enhancer and shipped with a cold pack and stored at 4°C upon arrival to their destination for up to 1 week post-fixation.
Long Term Storage:
Fixed single cell/nuclei samples resuspended in Quenching Buffer, Enhancer and 50% Glycerol can be shipped on dry ice and stored at -80°C upon arrival for up to 6 months post-fixation.
Note: Transporting fixed cell suspensions at 4°C and storing -80°C upon arrival for long term storage is not supported.
Refer to the appendix of the following Demonstrated Protocols when preparing fixed sample suspension for storage.
- Fixation of Cells & Nuclei for Chromium Fixed RNA Profiling
- Tissue Fixation & Dissociation for Chromium Fixed RNA Profiling
- Isolation of Cells from FFPE Tissue Sections for Chromium Fixed RNA Profiling
Products: Fixed RNA Profiling, Single Cell Gene Expression Flex