Question: How do I thaw Xenium Decoding Module B prior to a Xenium Analyzer run?
Answer: Decoding reagents are used for Xenium on-instrument analysis. As part of the 1 rxn / 1 instrument run kit, we will be providing two plates, Decoding Module A (PN-2000800) and Decoding Module B (PN-2000799). Decoding Module A (PN-2000800) is stored at 4C and should be kept at 4C until it is mixed and spun down. Decoding Module B (PN-2000799) must be thawed.
Decoding Module B Plate Preparation
- Decoding Module B is stored at -20°C prior to use.
- Thawing must be done in original mylar packaging. We recommend placing Decoding Module B at 4°C overnight with cardboard removed and mylar sleeves intact.
- Mylar packaging should remain sealed during thawing.
- Placing the mylar sealed plate with space around it (rather than touching or stacking) may help ensure the plate thaws at 4°C .
- Alternative thawing options are:
- 4°C over the weekend
- 37°C water bath for 2.5 hours
- Always verify that the reagent plate is fully thawed. If the plate is not thawed after 4°C overnight, place mylar package in a 37°C water bath briefly to thaw.
- Equilibrated 30 min at room temperature prior to loading.
- After equilibration, Decoding Module B must be mixed by inverting 20x and spun down prior to use.
- Centrifuge at 300 rcf for 1 min using a swinging bucket centrifuge with microplate adaptor at room temperature.
- Create a counterbalance for centrifugation.
- After opening mylar packaging for A/B, decoding module must be used on the same day.
- In extreme cases (e.g. run won't start), plates can be stored overnight at 4°C, but only with Support team approval.
Please consult the Xenium Analyzer User Guide (CG000584) for details on preparing the plates. Also What is the maximum number of freeze-thaw cycles for Xenium Decoding Module Plates A and B?
Products: Xenium Analyzer