Question: How do I thaw Xenium Decoding Module A/B prior to a Xenium Analyzer run?
Answer: Decoding reagents are used for Xenium on-instrument analysis. As part of the 1 rxn / 1 instrument run kit, we will be providing two plates, Decoding Module A (PN-2000800) and Decoding Module B (PN-2000799). These plates must be thawed prior to an instrument run.
Decoding Module Plate Preparation
- Plates are stored at -20°C prior to use.
- Thawing must be done in original mylar packaging. We recommend placing both Decoding Module A and Decoding Module B at 4°C overnight with cardboard removed and mylar sleves intact.
- Mylar packaging should remain sealed during thawing.
- Placing the mylar sealed plates with space around them (rather than touching or stacking) may help ensure reagents thaw at 4°C .
- Alternative thawing options are:
- 4°C over the weekend
- 37°C water bath for 2.5 hours
- After opening mylar packaging - must be used within 5 days (includes run time).
- Always verify that the reagent plates are fully thawed. If reagent plates are not thawed after after 4°C overnight, they can be placed in 37C water bath briefly to thaw.
- After thawing, reagents must be mixed by inverting 20x and spun down prior to use.
- Centrifuge at 300 rcf for 1 min using a swinging bucket centrifuge with microplate adaptor at room temp
- Module A is heavier than Module B
- Must weigh both modules and create a counterbalance for centrifugation
- Decoding module B - equilibrated 30 min at room temp prior to loading
Please consult the Xenium Analyzer User Guide (CG000584) for details on preparing the plates. Also What is the maximum number of freeze-thaw cycles for Xenium Decoding Module Plates A and B?
Products: Xenium Analyzer