Question: How can Xenium slides be stored prior to a Xenium Analyzer run?
Answer: Store cassettes sealed with a Xenium Cassette Lid as indicated in the Xenium In Situ Gene Expression - Probe Hybridization, Ligation & Amplification.
Cassettes with lids should always be stored hydrated with fresh PBS-T buffer at 4°C in the dark. After the assay workflow is completed, hydrated Xenium slides in cassettes can be stored for up to four days prior to Xenium Analyzer run. In limited testing, 24 days of storage may be possible with minimal impact on data quality generated. During this time, we recommend performing a PBS-T buffer exchange every ~4 days. The risk of storing slides for up to 24 days is that there may be a lower number of genes or transcripts detected per cell and tissue morphology may change over time. This is dependent on many factors including the input tissue quality, how nuclease-free the workflow is, and microbe-free the storage chamber is.
Slides can be stored after Xenium Analyzer run (or in the event of a run failure) in a similar manner. Please reach out to support@10xgenomics.com for more information.
Products: Xenium In Situ Gene Expression, Xenium Analyzer