Question - Why do I get an error of "invalid mkref version" with my custom reference in 10x Genomics Cloud Analysis?
Answer - Despite following the instructions for generating a custom reference using cellranger mkref, I am still encountering an error when I try to upload my reference package.
Verifying all required files are present...
Invalid request. refRaw: invalid mkref version detected "cellranger-cellranger-7.1.0"
Currently cloud analysis offers cellranger pipelines in versions v3.1 through v7.0.1 as listed on our supported products and pipelines page.
The latest version of cellranger is v7.1. This version of cellranger is available for download and use with any system that meets our system requirements. However, it is not yet available on 10x cloud analysis. As this is a newer version than what is currently supported in cloud analysis, it will not be possible to upload references generated using cellranger mkref v7.1 due to version incompatibility. In order to use custom references on cloud analysis, the version of mkref used to create the reference has to be 7.0.1 or older.
Here is the page to download cellranger v7.0.1 to run mkref that will be compatible with the 10x cloud.
Please contact support@10xgenomics.com if you encounter issues with reference uploads.
10x Genomics Cloud Analysis is only available in the United States and Canada.