Question: What is the difference between the imageable area and the sample positioning area on Xenium slides?
Answer: Xenium slides include an imageable area outlined by a white line with a break in the center of the line. This area measures 12 mm x 24 mm.
Within the imageable area is the available sample positioning area or sample area for short. This is the region outlined by fiducials (‘+’) on the Xenium slide and measures 235 mm2 (10.45 mm x 22.45 mm).
Sample are placed within the fiducials as shown here with three mouse brain sections:
Fiducial registration is critical for instrument performance, so it is important not to cover the fiducials during tissue placement.
Products: Xenium In Situ Gene Expression