Question: When should I start the 10 minute lysis timer for the Nuclei Isolation Kit?
Answer: Most tissues will take 30-40 seconds to mechanically dissociate, but tough tissues (such as heart, muscle, and fibrous tissue) may take up to two minutes to fully dissociate with the plastic pestle. We recommend starting the 10 minute lysis timer after the mechanical dissociation step.
Products: Single Cell Gene Expression, Single Cell ATAC, Single Cell Multiome ATAC + GEX, Fixed RNA Profiling