Question: Can I substitute MilliQ water for nuclease-free water in the Xenium workflow?
Answer: At many steps in both the Xenium In Situ Gene Expression assay and Xenium Analyzer workflow, nuclease-free water (Thermo Fischer Scientific, AM9937*) is a required third-party reagent. It is not possible to substitute MilliQ** water for nuclease-free water. The Xenium workflow has been carefully designed to maintain RNA integrity and this reagent swap may compromise assay performance.
*Alternate brands of nuclease-free water are low-risk reagent swaps: Can I use alternative third-party reagents for Xenium?
**It is possible to substitute nuclease-free (not DEPC-treated) water for any steps that calls for MilliQ water.
Products: Xenium In Situ Gene Expression