Question: Is Immunofluorescence (IF) staining compatible with the Visium CytAssist Spatial Gene Expression for Fresh Frozen or Fixed Frozen assays?
Answer: We have not validated immunofluorescence staining with either assay. The Visium CytAssist Spatial Gene Expression for Fresh Frozen and Fixed Frozen assays are compatible with H&E Staining only.
The Visium CytAssist Spatial Gene Expression for FFPE assay is compatible with IF stained tissues. Additional details can be located in the following demonstrated protocol:
Product: Visium CytAssist for FF, Visium CytAssist for FxF