Question: Is Immunofluorescence (IF) staining compatible with the Visium CytAssist Spatial Gene Expression for Fresh Frozen or Fixed Frozen assays?
Answer: We have not validated immunofluorescence staining with either assay. The Visium CytAssist Spatial Gene Expression for Fresh Frozen and Fixed Frozen assays only support H&E Staining.
Based on some limited testing performed, Fixed Frozen (FxF) mouse tissue samples can be compatible with IF staining. We have described the workflow steps and modifications required to successfully execute IF staining with mouse FxF samples in this article:
Please note that the modifications to the Demonstrated Protocols and the User Guides listed in the above article have not been fully validated and are considered unsupported. Customers will need to proceed at their own risk.
The Visium CytAssist Spatial Gene Expression for FFPE assay is compatible with IF stained tissues. Additional details can be located in the following demonstrated protocol:
Product: Visium CytAssist for FF, Visium CytAssist for FxF