Question: What section thickness is recommended for use with the Visium CytAssist for Fresh Frozen solution?
Answer: Based on internal testing with sections 5 - 30 μm, we learned that similar results were achieved with sections 10 μm and 20 μm in thickness. 5 µm sections resulted in lower sensitivity (lower Genes and UMI counts per tissue covered spot) and >20 µm sections resulted in a loss of spatiality (lower reads in spots covered with tissue). Therefore, we strongly recommend 10 - 20 μm sections.
Products: Visium CytAssist for FF