Question: How do I clean the bottles used to prepare Xenium Analyzer Buffers?
Answer: Pyrex Reusable Media Storage Bottles (500 ml and 1 L) or equivalent are used to prepare buffer for Xenium Analyzer instrument operation. Here are brief guidelines for cleaning residual Xenium Reagent Buffers from the glass bottles used during buffer preparation. This applies to the glass bottles used to prepare both Xenium Sample Wash Buffer A (Bottle 2) and Xenium Probe Removal Buffer (Bottle 4). The guidelines below are recommendations and alternative cleaning protocols may be followed. The most critical factors to consider for any bottle cleaning are that the bottles after cleaning are free of residual detergents, free of debris, and nuclease activity is minimized.
Bottle Cleaning Procedure
Equipment and Materials
- Deionized water
Bottle cleaning suggestions:
- Rinse bottle 3x with deionized water
- Dry bottle in clean area, preventing particles/debris from falling inside
- Recap the washed and dried bottle
- Autoclave (make sure cap loose)*
*If an autoclave is not available, rinse bottle 1x with nuclease-free water prior to use
Product: Xenium In Situ Gene Expression