Question: Can different panels be run sequentially on the same tissue section?
Answer: During probe hybridization (see Xenium In Situ Gene Expression - Probe Hybridization, Ligation & Amplification for more details), pre-designed DNA probes, alone or in combination with custom DNA probes, are added to the FFPE or FF tissue sections. These probes contain gene specific barcodes that are imaged during the Xenium Analyzer run and the gene identity is defined based on the Xenium Analyzer images and a proprietary codebook. Taking sections with previously hybridized probes through a second round of hybridization will yield noisy data and an inability to decode genes due to the presence of gene specific barcodes from each workflow and overlap in codewords.
Products: Xenium In Situ Gene Expression