How should I prepare my Liberase TH solution for the Isolation of Cells from FFPE Tissue Sections for Chromium Fixed RNA Profiling Demonstrated Protocol?

Question: How should I prepare my Liberase TH solution for the Isolation of Cells from FFPE Tissue Sections for Chromium Fixed RNA Profiling Demonstrated Protocol?

Answer: The Liberase TH solution should be prepared by performing the following steps:

1. Reconstitute 5 mg of Liberase TH by adding 1 mL sterile Thermo Fisher Scientific nuclease-free water (catalog number: AM9937)
   
2. Agitate solution at 2-8°C until completely dissolved
   
3. Store stock solution in single-use aliquots at -15°C to -25°C
   

We are currently updating our documentation to include these instructions.

Products: Fixed RNA Profiling Gene Expression